Table 1.
Summary of transport kinetics for live tracking of synaptic vesicles (Syt-1-GFP) and dense-core vesicles (ANF-GFP) expressed with the CCAP-GAL4 driver.
| Anterograde
|
Retrograde
|
|||||
|---|---|---|---|---|---|---|
| Control | dhtt null | dhtt rescue | Control | dhtt null | dhtt rescue | |
| Synaptic vesicles | ||||||
| Flux (particles/min) | 16 ± 4.0 | 12* ± 3.2 | 17 ± 3.7 | 6.1 ± 1.5 | 8.3* ± 1.0 | 6.2 ± 1.6 |
| Run distance (µm) | 14.1 ± 2.1 | 10.2* ± 1.8 | 15.7 ± 2.5 | 7.6 ± 1.2 | 7.8 ± 1.1 | 7.9 ± 1.9 |
| Run Duration (s) | 12.7 ± 1.1 | 7.8* ± 1.0 | 11.5 ± 1.9 | 9.1 ± 0.6 | 8.0 ± 1.0 | 8.6 ± 1.7 |
| Velocity (µm/s) | 0.85 ± 0.09 | 0.82 ± 0.08 | 0.86 ± 0.08 | 0.70 ± 0.08 | 0.74 ± 0.07 | 0.66 ± 0.07 |
| Stops (per min) | 5.1 ± 1.1 | 8.0* ± 1 | 4.1 ± 0.9 | 6.5 ± 0.9 | 8.2 ± 0.9 | 7.0 ± 0.9 |
| Reversals (per min) | 2.3 ± 0.9 | 2.3 ± 0.8 | 2.2 ± 0.8 | 8.2 ± 2.1 | 8.3 ± 1.9 | 7.9 ± 1.7 |
| Dense-core vesicles | ||||||
| Flux (particles/min) | 22 ± 1.8 | 18* ± 1.7 | 21 ± 2.2 | 8.1 ± 1.0 | 11* ± 2.1 | 7.6 ± 1.3 |
| Run distance (µm) | 12 ± 1.7 | 11 ± 1.2 | 11 ± 1.4 | 10 ± 1.1 | 11 ± 1.4 | 9.8 ± 1.2 |
| Run duration (s) | 8.1 ± 1.0 | 7.1 ± 0.9 | 8.7 ± 1.4 | 7.2 ± 1.5 | 6.3 ± 0.9 | 6.1 ± 1.4 |
| Velocity (µm/s) | 0.75 ± 0.08 | 0.77 ± 0.08 | 0.72 ± 0.1 | 0.69 ± .07 | 0.72 ± .07 | 0.64 ± .07 |
| Stops (per min) | 6.1 ± 0.4 | 6.0 ± 0.3 | 7.0 ± 0.5 | 7.2 ± 0.5 | 6.5 ± 0.4 | 7.0 ± 0.5 |
| Reversals (per min) | 2.2 ± 0.8 | 2.3 ± 0.5 | 2.5 ± 0.7 | 5.5 ± 0.5 | 5.1 ± 0.5 | 5.9 ± 0.5 |
A region of bleached axon was allowed to recover for 60s and GFP-tagged organelles were tracked manually as they entered the bleached region. N > 100 particles for each genotype.
Error bars represent SEM, *indicates p < 0.05 compared to control using Student’s t-test.