Fig. 1.

Three transgenic mice with different PKCγ mutations. a We generated three different PKCγ mutated transgenic mouse lines (named PKC-A, PKC-B and PKC-C). The genotype of the founders was confirmed by PCR. b Western blot analysis of total PKCγ protein in the cerebellum from each transgenic mouse. (−) Non transgenic mice, (+) Transgenic mice. c Quantitative RT–PCR confirmed that all transgenic lines express human PRKCG which encode PKCγ protein. d Purkinje cells in organotypic slice cultures from each transgenic mouse line are shown. Anti-calbindin staining showing all Purkinje cells and anti-GFP immunostaining is shown for transgene expression. Scale bar in H = 50 μm. e Each Purkinje cell area was calculated with image J and analyzed with Graphpad prism. Data are shown as the mean ± S.D. of at least 20 Purkinje cells ****p < 0.0001