Fig. 2.

Morphology of Purkinje cells from PKCγ mutated transgenic mice in dissociated primary culture. The morphology of Purkinje cells was analyzed after 2 weeks in culture. a Wildtype (WT) Purkinje cells showed normal dendritic expansion in dissociated primary culture. DMSO treated Purkinje cells showed normal dendritic growth. PMA (15 nM) treated Purkinje cells showed inhibition of dendritic growth. Purkinje cells from PKC-A transgenic mice showed normal dendritic growth. Purkinje cells from PKC-B transgenic mice showed normal dendritic growth. Purkinje cells from PKC-C transgenic mice showed inhibition of dendritic growth. Scale bar = 50 μm. b Quantification of the Purkinje cell area. The dendritic tree size of Purkinje cells in PMA treated cultures or cultures from S361G transgenic mice was strongly reduced. Each Purkinje cell area was calculated with image J and analyzed with Graphpad prism. Data are shown as the mean ± S.D. of at least 20 Purkinje cells ****p < 0.0001