Fig. 5.

Morphology of Purkinje cells transfected with PKCγ catalytic domain mutants. The morphology of Purkinje cells was analyzed after 2 weeks in culture. a Purkinje cells transfected with GFP control vector. b Purkinje cells transfected with PKCγ WT. c Purkinje cells transfected with PKCγ-G360S. d Purkinje cells transfected with the PKCγ-S361G (e) Purkinje cells transfected with PKCγ-F643 L. Scale bar in E = 50 μm. f Quantification of the Purkinje cell area. The dendritic tree size of Purkinje transfected with the catalytic domain mutations S361G (P < 0.0001) or F643 L (P < 0.0025) was significantly reduced. Data are shown as the mean ± S.D. of at least 40 Purkinje cells. The value for Purkinje cells transfected with PKCγ WT was set as 100%