Table 5.
PRP binding to hydrolyzable vs. condensed tannins
| Reference | Method | Tannin type | Bond stability | Binding affinity |
|---|---|---|---|---|
| [66] | Competitive binding assay | 5GG, gallic acid, EGC | nd | Hydrolyzable tannin > condensed tannin |
| [62] | Competitive binding assay | Quebracho and tannic acid | Hydrolyzable: 20% greater dissolution of bonds in gastric and enteric digestion | Hydrolyzable tannin > condensed tannin |
| [48] | NMR | B2, PGG, TGG, PAC monomer | Hydrogen bonds associated with hydrolyzable tannins | B2 > PGG > TGG> |
| [47] | NMR, DLS | EgCG, EGC, PGG | Hydrogen bonds associated with hydrolyzable tannins | Hydrolyzable tannin > condensed tannin |
| [63] | SDS PAGE | Wine or tannic acid | Condensed tannins associated with hydrophobic bonds | nd |
| [61] | HPLC | Hydrolyzable vs. condensed wine extracts | nd | Increased precipitation of condensed tannin at pH 7.5 c/t hydrolyzable tannin |
NMR Nuclear magnetic resonance imaging, DLS dynamic light scattering, SDS-PAGE sodium dodecyl polyacrylamide gel electrophoresis, HPLC high performance liquid chromatography. B2: proanthocyanidin B2, PGG pentagalloylglucose, TGG tetragalloylglucose, PAC proanthocyanidin, EgCG epigallocatechin gallate, ECG epigallocatechin, PRP proline rich protein. nd = not determined