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. Author manuscript; available in PMC: 2017 Jul 25.
Published in final edited form as: Cytotherapy. 2013 Mar;15(3):255–262. doi: 10.1016/j.jcyt.2012.11.013

Figure 1. Basic steps for setting up the CFU assay.

Figure 1

Step 1) An aliquot of cells is removed from a stem cell product; Step 2) A pre-dilution cell count and viability test are performed; Step 3) A working cell suspension is made from the aliquot of cells removed from step 1; Step 4) Semi-solid medium containing growth factors is inoculated with a defined volume of the working cell suspension; Step 5) The semi-solid medium containing growth factors and cells are transferred to a culture vessel; Step 6) The culture vessel is placed in an incubator for a defined period of time; Step 7) At the end of the culture period, the colonies are enumerated and differentiated.