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. 2017 Jul;58(9):3723–3731. doi: 10.1167/iovs.17-21781

Figure 1.

Figure 1

Characterization of Slc4a11+/+ and Slc4a11−/− MCECs. (A) Genotypes of mice used for corneal endothelial cell collection, and genotypes of cultured MCECs. Slc4a11 wild-type (wt) and knockout (ko) alleles were identified with specific primers yielding products of 353 bp and 386 bp, respectively. Immorto (tsTAg) and null alleles yielded PCR products of 500 bp and 300 bp, respectively. Slc4a11+/+ and Slc4a11−/− mice with one allele of Immorto gene were used for cell derivation. Slc4a11+/+ and Slc4a11−/− MCECs show the expected genotype of Slc4a11 and tsTAg gene. (B) RT-PCR of Slc4a11+/+ and Slc4a11−/− MCECs verified that no Slc4a11 mRNA is expressed in ko. (C) Morphology of Slc4a11+/+ and Slc4a11−/− MCECs cultured at 33°C with and without IFN-γ. (D) Growth curve of Slc4a11+/+ and Slc4a11−/− MCECs cultured at 33°C with and without IFN-γ.