Overexpression of PAX6 had no effect on the migration or invasion of MCF-7 cells. (A) Wound scratch assay and (B) Transwell migration assay were conducted to examine the cell migratory and invasive capacities of MCF-7 cells transfected with PAX6 plasmid. PAX6, paired box 6; MCF, Michigan Cancer Foundation; Control, non-transfected MCF-7 cells. For wound scratch assay, a magnification of ×40 was used. For transwell assay, a magnification of ×400 was used.