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. 2017 Jun 12;14(2):1253–1259. doi: 10.3892/etm.2017.4580

Figure 2.

Figure 2.

Gln treatment promotes α-Syn degradation in PC12 (α-Syn+) cells. (A) Levels of α-Syn mRNA in PC12 (α-Syn+) cells or in PC12 (con) cells, following an incubation for 6, 12 or 24 h. (B) Western blot analysis of α-Syn and Hsp70 protein levels in PC12 (α-Syn+) or PC12 (Con) cells, following an incubation time for 12, 24 or 48 h. Relative levels of (C) α-Syn or (D) Hsp70 in PC12 (α-Syn+) or PC12 (Con) cells, following an incubation time for 12, 24 or 48 h. (E) Relative levels of α-Syn mRNA in the PC12 (α-Syn+) cells with or without 10 mM Gln treatment for 6, 12 or 24 h. (F) Western blot analysis of α-Syn protein levels in the PC12 (α-Syn+) cells, following the treatment with 0, 5, 10 or 20 mM Gln for 24 h. All results are presented as mean ± standard deviation for triple experiments. *P<0.05, **P<0.01, ***P<0.001 vs. control. Gln, glutamine; α-Syn, α-Synuclein; PC12 (α-Syn+), α-Syn-overexpressed PC12; con, control; Hsp, heat shock protein; H. P. T., h post transfection; H, h.