Figure 4.

Gln reduces the proteasome inhibitor-promoted α-Syn accumulation in neuroblastoma PC12 cells. (A) Levels of α-Syn mRNA in PC12 (α-Syn+) cells treated with 10 mM Gln and/or 5 µM Lac for 6, 12 or 24 h. (B and C) Western blot analysis of α-Syn and Hsp70 in PC12 (α-Syn+) cells treated with 0, 2, 5 or 10 µM Lac for 24 h. (D and E) Western blot analysis of α-Syn in PC12 (α-Syn+) cells treated with 2 or 5 µM Lac and/or 5 or 10 mM Gln for 24 h. All experiments were performed in triplicate and data are presented as the mean ± standard deviation. *P<0.05, **P<0.01 vs. 0 µM Lac. Gln, glutamine; α-Syn, α-Synuclein; PC12 (α-Syn+), α-Syn-overexpressed PC12; Lac, lactacystin; Hsp, heat shock protein.