Figure 4.

Matrix related protein expression of NP cells after CY treatment. Western blotting used to measure aggrecan and collagen II protein expression of NP cells. Cells were cultured with different concentrations of CY and/or 1 µg/ml LPS for 5 or 8 days, then total protein of cells were extracted and performed western blotting. (A and C) Expression of collagen II and aggrecan at 5 and 8 days. (B and D) Positive immunoreactive bands were densitometrically quantified and normalized to GAPDH. The data are presented as the means ± SD. ***P<0.001, **P<0.01. All data were obtained from at least three independent experiments.