Table I.
Gene | Primer sequences (5′-3′) |
---|---|
TNF-α | Forward: GGCTTTCGGAACTCACTGGA |
Reverse: GGGAACAGTCTGGGAAGCTC | |
Collagen II | Forward: GGCCAGGATGCCCGAAAATTA |
Reverse: ACCCCTCTCTCCCTTGTCAC | |
Aggrecan | Forward: CAGATGGCACCCTCCGATAC |
Reverse: GACACACCTCGGAAGCAGAA | |
ADAMT-4 | Reverse: ACCGATTACCAGCCTTTGGG |
Forward: CCGACTCCGGATCTCCATTG | |
ADAMT-5 | Forward: CCGAACGAGTTTACGGGGAT |
Reverse: TGTGCGTCGCCTAGAACTAC | |
MMP3 | Forward: TTTGGCCGTCTCTTCCATCC |
Reverse: GCATCGATCTTCTGGACGGT | |
GAPDH | Forward: TGCCACTCAGAAGACTGTGG |
Reverse: TTCAGCTCTGGGATGACCTT |