Protective effect of glycyrrhizin on AST, LDH, ALT and CK in rats with myocardial I/R injury. The protective effect of glycyrrhizin was investigated by determining the levels of (A) AST, (B) LDH, (C) ALT and (D) CK in rats with myocardial I/R injury. Rats were divided into five groups: Sham, NS and myocardial I/R injury + pre-treatment with 2, 4 and 10 mg/kg glycyrrhizin groups, respectively, to investigate the effect of glycyrrhizin on infarct size in myocardial I/R injury. Data are presented as the mean ± standard deviation. **P<0.01 vs. Sham; ##P<0.01 vs. NS. I/R, ischemia/reperfusion; AST, aspartate aminotransferase; LDH, lactate dehydrogenase; ALT, alanine aminotransferase; CK, creatine kinase; Sham, sham-treated group; NS, myocardial I/R injury + non-treated group; 2 mg/kg, myocardial I/R injury + pre-treatment with 2 mg/kg glycyrrhizin group; 4 mg/kg, myocardial I/R injury + pre-treatment with 4 mg/kg glycyrrhizin group; 10 mg/kg, myocardial I/R injury + pre-treatment with 10 mg/kg glycyrrhizin group.