Figure 3.

Effects of L1 RNA binding to PSF on GAGE6. (A) Binding of L1 RNA releases GAGE6 promoter DNA from PSF. (B) Release of GAGE6 promoter region from PSF by overexpression of L1 RNA demonstrated by chromatin immunoprecipitation. (C) Expression levels of PSF mRNA, L1 RNA and GAGE6 mRNA in A549 and 16HBE cells transfected with L1 RNA overexpression or empty vector measured using reverse-transcription quantitative polymerase chain reaction. *P<0.05, **P<0.01, ***P<0.001. GAGE6, G antigen 6; 5′UTR, 5′ untranslated region; Ab, antibody; IgG, immunoglobulin G; E. coli, Escherichia coli; L1, long interspersed nuclear element 1; PSF, polypyrimidine tract-binding protein-associated splicing factor; DHFR, dihydrofolate reductase.