Frequency of CD27+IgM+B cells in patients with CHC and HCs. (A) Gating strategy for IgM+B cell subsets by flow cytometry. (B) Frequencies of IgM+B, CD27-IgM+B and CD27+IgM+B cells in total B cells. *P<0.05. PerCP, peridinin chlorophyll; PE, phycoerythrin; CHC, chronic hepatitis C; HC, healthy control; NS, no significant difference; FSC, forward scatter; SSC, side scatter; Ig, immunoglobulin; FITC, Fluorescein isothiocyanate; CD, cluster of differentiation.