Abnormal differentiation of IgM+B cell subsets in patients with CHC. (A) Gating strategy for measuring the differentiation of IgM+B cell subsets by flow cytometry. (B) Frequency of CD27-CD10+B cells in IgM+B cells. (C) Frequency of IgD+B cells in IgM+B cell subsets. (D) Frequency of CD38+B cells in IgM+B cell subsets. *P<0.05. CHC, chronic hepatitis C; HC, healthy control; NS, no significant difference; PE, phycoerythrin; PerCP, peridinin chlorophyll; Ig, immunoglobulin; CD, cluster of differentiation; APC, allophycocyanin.