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. 2017 Jun 15;7(9):2377–2391. doi: 10.7150/thno.19408

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In a second trauma model, the thoracic spine between T7 and T8 is punctured with a 25G needle. [^18/19F]-2 is intrathecally injected, post-trauma, and used to identify the site of spinal cord puncture that is not visible by (i) horizontal and (ii) sagittal CT. PET/CT imaging of intrathecal [^18/19F]-2 show the leak site between T7 and T8 in (iii) maximum intensity projection PET (red), (iv) horizontal PET/CT, and (v) sagittal PET/CT. (B) Superior to inferior, axial 2.5 mm PET/CT sections of intrathecal [^18/19F]-2 in the thoracic spine show a clear leak between T7 and T8. (C) The PET overlay is removed. Spinal defects that would indicate the site of puncture are not observable in CT. The puncture site is not obvious in CT. 4 rats were used in this study. (Additional replicates in supporting information S15, S16)