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. 2017 Jun 24;7(9):2452–2462. doi: 10.7150/thno.18813

Figure 2.

Figure 2

miR-196a suppresses the expression of RANBP10 through binding to 3'UTR of RANBP10. N2a cells were co-transfected with miR-196a and a reporter construct to determine the direct binding of miR-196a on 3'UTR of RANBP10. N2a cells transfected with miR-196a and the brains of miR-196a transgenic mice were used to determine the suppression of endogenous RANBP10. (A) Wild-type or mutant (mut.) 3'UTRs of RANBP10 was constructed into the 3' end of luciferase gene for the reporter assay. (B) Luciferase reporter assay shows miR-196a binds to 3'UTR of RANBP10 to suppress the expression of luciferase activity compared to those in the miR-196a non-relative control (NC) or mutant 3'UTR of RANBP10 (Mut. RANBP10 3'UTR). N=3. (C) Western blotting shows the expression of RANBP10 after treatment of miR-196a mimics and NC in N2a cells. (D) Quantitation results show the suppression of RANBP10 after treatment of miR-196a mimics in N2a cells. N=8. (E) Western blotting shows the expression of RANBP10 in the brains of miR-196a transgenic and non-transgenic (NTG) mice. (F) Quantitation results show the lower expression of RANBP10 in miR-196a transgenic mice. *indicates a significant difference with P<0.05. **indicates a significant difference with P<0.01.