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. 2017 Jun 21;7(2):173–178. doi: 10.3892/br.2017.931

Figure 2.

Figure 2.

Stress condition effect on the expression of IL-24/mda-7 in LX-2 cells. (A) Serum starvation and leptin treatment increase IL-24/mda-7 mRNA expression level. The mRNA expression of IL-24/mda-7 in normal and activated cell was analyzed by reverse transcription-quantitative polymerase chain reaction on 24 h. (B) An increase in the amount of IL-24/mda-7 protein by ELISA on 24 and 48 h following stress condition was observed. Data (mean ± standard error of the mean) are shown. The analysis of ELISA and reverse transcription-quantitative polymerase chain reaction demonstrated that leptin and serum starvation obviously increased the expression of IL-24/mda-7. *P<0.05, **P<0.01 vs. control. IL, interleukin; mda, melanoma differentiation associated gene.