Figure 2.
A. Schematic view of the TEER-chip and 4-point impedance measurement chosen to measure TEER and capacitance. A small current of 10 μA of varying frequency is applied between two electrodes (Iexcite) located on each side of the cell culture, and the drop in potential between the second set of electrodes measured (Vmeas). B. Example of impedance spectra recorded before ALI and after full differentiation at ALI. Lines are fitted data based on the equivalent electronic circuit used for modelling.