|
| ||
| Component | Amount (μl) | Final concentration |
|
| ||
| Tris-HCl, pH 7.5, 1M | 250 | 50 mM |
| CaCl2, 1M | 50 | 10 mM |
| Glycerol | 2500 | 50% (vol/vol) |
| UltraPure Water | 2200 | |
| Total | 5000 | |
|
| ||
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