|
| ||
| Component | Amount per reaction (μl) | Final concentration |
|
| ||
| FastDigest Buffer, 10× | 2 | 1× |
| Library Plasmid Backbone | 1 | 50 ng μl−1 |
| FastDigest Esp3I (BsmBI) | 1 | |
| FastAP Thermosensitive Alkaline Phosphatase | 1 | |
| DTT, 100 mM | 0.2 | 1 mM |
| UltraPure water | 14.8 | |
| Total | 20 | |
|
| ||
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