|
| ||
| Component | Amount per reaction | Final concentration |
|
| ||
| Gibson Assembly Master Mix, 2× | 10 μl | 1× |
| Digested library Plasmid Backbone from Step 10 | 330 ng | 16.5 ng μl−1 |
| SgRNA library insert from Step 7 or UltraPure water control | 50 ng | 2.5 ng μl−1 |
| UltraPure water | To 20 μl | |
| Total | 20 μl | |
|
| ||
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