|
| ||
| Component | Amount per reaction (μl) | Final concentration |
|
| ||
| Gibson Assembly Reaction | 20 | |
| Isopropanol | 20 | |
| GlycoBlue Coprecipitant | 0.2 | 0.075 μg μl−1 |
| NaCl solution, 5 M | 0.4 | 50 mM |
| Total | ~40 | |
|
| ||
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