Flag	Description	Default
-f	Fastq file containing NGS data for analysis	NGS.fastq
-o	Output csv file with guide spacer sequences in the first column and respective read counts in the second column	library_count.csv
-i	Input csv file with guide spacer sequences	library_sequences.csv
-no-g	Indicate absence of a guanine before the guide spacer sequence	guanine is present