|
| ||
| Component | Amount (μl) | Final concentration |
|
| ||
| sgRNA-top, 100 μM | 1 | 10 μM |
| sgRNA-bottom, 100 μM | 1 | 10 μM |
| T4 ligation buffer, 10× | 1 | 1× |
| T4 PNK | 0.5 | |
| UltraPure water | 6.5 | |
| Total | 10 | |
|
| ||
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