|
| ||
| Component | Amount (μl) | Final concentration |
|
| ||
| KAPA HiFi HotStart ReadyMix, 2× | 10 | 1× |
| First round PCR from Step 91 | 1 | |
| NGS-indel-R2-Fwd | 1 | 0.5 μM |
| NGS-indel-R2-Rev | 1 | 0.5 μM |
| UltraPure water | 7 | |
| Total | 20 | |
|
| ||
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