|
| ||
| Component | Amount (μl) | Final concentration |
|
| ||
| KAPA HiFi HotStart ReadyMix, 2× | 10 | 1× |
| QuickExtract from Step 89 | 1 | |
| NGS-indel-R1-Fwd | 1 | 0.5 μM |
| NGS-indel-R1-Rev | 1 | 0.5 μM |
| UltraPure water | 7 | |
| Total | 20 | |
|
| ||
|
| ||
| Component | Amount (μl) | Final concentration |
|
| ||
| KAPA HiFi HotStart ReadyMix, 2× | 10 | 1× |
| QuickExtract from Step 89 | 1 | |
| NGS-indel-R1-Fwd | 1 | 0.5 μM |
| NGS-indel-R1-Rev | 1 | 0.5 μM |
| UltraPure water | 7 | |
| Total | 20 | |
|
| ||