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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Nat Protoc. 2017 Mar 23;12(4):828–863. doi: 10.1038/nprot.2017.016

Flag Description Default

-f Indicates input file is fasta format Fastq file format
-a Uses alternative hashing algorithm for calculation74 Ratcliff-Obershelp based algorithm73
-o Output file with calculated indel rates and statistics calc_indel_out.csv
-i Input file with sample name, fasta or fastq file name, guide sequence, PCR target amplicon, and experimental or control in columns from left to right sample_sheet.csv
-v or -q Increase or decrease reporting as script runs Standard reporting
-no-m Does not perform MLE correction MLE correction is performed