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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Nat Protoc. 2017 Mar 23;12(4):828–863. doi: 10.1038/nprot.2017.016
Component Amount (μl) Final concentration
Reaction Buffer, 5× 5 1×
dNTP Mix, 10 mM 1.25 0.5 mM
Random Hexamer Primer, 100 μM 1.09 4.4 μM
Oligo dT, 100 μM 0.88 3.5 μM
RiboLock RNAse Inhibitor, 40 U μl−1 0.125 0.2 U μl−1
RevertAid Reverse Transcriptase, 200 U μl−1 1.25 10 U μl−1
UltraPure water 10.41
Total 20