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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Nat Protoc. 2017 Mar 23;12(4):828–863. doi: 10.1038/nprot.2017.016
Component Amount (μl) Final concentration
TaqMan Fast Advanced Master Mix, 2× 12 1×
TaqMan Gene Expression Assays for candidate gene, 20× 1.2 1×
TaqMan Gene Expression Assays for control gene, 20× 1.2 1×
cDNA 9.6
Total 24