Flag	Description	Default
-o	Output csv file with names for target genes, spacer sequences, spacer orientations, chromosome locations, cleavage site locations, off-target scores, and oligo library sequences in columns from left to right	final_guides.csv
-i	Prefix of input genome 2bit file	hg19
-g	Target genes csv file with the gene name, chromosome, start of the targeted region, and end of the targeted region in columns from left to right	genes.csv
-gc	Minimum GC content required for an sgRNA spacer sequence	25
-s	Minimum spacing required between cleavage sites of sgRNAs targeting the same genomic region	20
-n	Maximum number of guides selected targeting each gene in the target genes csv file	3
-db	Use an existing off-target database constructed from a previous custom library design for a new library	False
-gecko or -sam	Specify the type of library and add the respective flanking sequences to the spacers for the oligo library synthesis	Neither