|
| ||
| Component | Amount per reaction (μl) | Final concentration |
|
| ||
| NEBNext High Fidelity PCR Master Mix, 2× | 12.5 | 1× |
| Pooled oligo library template from Step 2 | 1 | 0.04 ng μl−1 |
| Oligo-Fwd primer (Universal) | 1.25 | 0.5 μM |
| Oligo-Knockout-Rev or Oligo-Activation-Rev primer | 1.25 | 0.5 μM |
| UltraPure water | 9 | |
| Total | 25 | |
|
| ||
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.