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. 2017 Jun 13;14(2):1212–1218. doi: 10.3892/etm.2017.4589

Figure 1.

Figure 1.

Effect of Cx43 expression on GJC function in colorectal carcinoma cell lines. (A) Western blot analysis of Cx43 expression in LoVo, HCT106 and HCT116 cell lines transfected with Cx43. ATPase β3 subunit served as a loading control. (B) Cx43 expression level was analyzed using Quantity One software (v4.62) and normalized against ATPase β3 expression level. Parachute dye-coupling assay results of the (C) LoVo, (D) HCT106 and (E) HCT116 cell lines. Scale bars represent 10 µm. (F) Quantified results of parachute dye-coupling assays in the three cell lines. Columns show the mean ± standard deviation from five independent experiments. *P<0.05 vs. the respective WT group. WT, wild type; Cx43, connexin 43; GJC, gap junctional communication.