Figure 2.

Expression of genes associated with alternatively activated macrophages in the lungs of OVA-induced asthmatic mice. Lung IMs from OVA-induced asthmatic mice and PBS-treated control mice were sorted 24 h after the final OVA challenge (day 27 post-model establishment; 100 µg in 50 µl PBS) and the levels of (A) Arg1, (B) TG2, (C) IL-10 mRNA and (D) iNOS mRNA, as markers of classically activated macrophages, were measured by reverse transcription-quantitative polymerase chain reaction. Expression of Arg1, TG2, iNOS, and IL-10 was normalized to GAPDH expression. Data are presented as the mean ± standard deviation, n=6. *P<0.05 and **P<0.01 vs. control. OVA, ovalbumin; IM, interstitial macrophage; Arg1, arginase 1; TG2, transglutaminase 2; iNOS, inducible nitric oxide synthase; IL-10, interleukin-10; PBS, phosphate-buffered saline.