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. 2017 Jun 23;14(2):1419–1426. doi: 10.3892/etm.2017.4655

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Copyright: © Ma et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Reversal experiment to determine the effect of miR-19a knockdown on TGFβR2. (A) Western blotting was used to examine the protein expression level of TGFβR2 in C666-1 cells transfected with miR-19a inhibitor, or co-transfected with miR-19a inhibitor and TGFβR2 siRNA, respectively, relative to GADPH. Following this, (B) MTT and (C) Transwell assays were used to determine cell viability and invasion capabilities, respectively. Data are presented as the mean ± standard deviation. **P<0.01 vs. miR-19a inhibitor. miR, microRNA; TGFβR2, transforming growth factor β receptor 2; siRNA, small interfering RNA; OD, optical density.