Effects of DHA on the viability and proliferation of renal cancer cells. (A) An MTS assay was performed to determine the viability of Caki-1 and 786-O cells following treatment with 25, 50 and 100 µM DHA for 24 h. Cell viability was significantly decreased in Caki-1 cells treated with 50 and 100 µM DHA and 786-O cells treated with 100 µM. (B) Total cell counts as a measure of proliferation were determined in Caki-1 and 786-O cells following treatment with 25, 50 and 100 µM DHA for 24, 48 and 72 h. Proliferation was significantly lower in both cell lines at all doses of DHA relative to controls at corresponding time points. #P<0.01 vs. untreated control cells of respective cell lines. DHA, docosahexaenoic acid; Caki-1 and 786-O, renal cancer cell lines.