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. 2017 Jun 26;14(2):1727–1731. doi: 10.3892/etm.2017.4669

Figure 1.

Figure 1.

eNOS overexpression in vitro was validated by reverse transcription-quantitative polymerase chain reaction and western blot analysis. (A) mRNA level of eNOS after pcDNA3.0-eNOS transfection for 24 h, compared with the negative control group (pcDNA3.0); (B) protein level of eNOS following pcDNA3.0-eNOS transfection for 24 h, compared with the negative control group. All detections were repeated independently three times. ***P<0.001 vs. the negative control group. eNOS, endothelial nitric oxide synthase.