Skip to main content
. 2017 Jun 26;14(2):1727–1731. doi: 10.3892/etm.2017.4669

Figure 2.

Figure 2.

Effect of overexpressed eNOS on SIRT1 expression in vitro was detected by reverse transcription-quantitative polymerase chain reaction and western blot analysis. (A) mRNA level of SIRT1 after pcDNA3.0-eNOS transfection for 24 h, compared with the negative control group (pcDNA3.0); (B) protein level of SIRT1 following pcDNA3.0-eNOS transfection for 24 h compared with the negative control group. All detections were repeated independently three times. ***P<0.001 vs. the negative control group. eNOS, endothelial nitric oxide synthase; SIRT1, sirtuin 1.