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Interaction between eNOS and SIRT1. Min6 cells were treated with pcDNA3.0-eNOS for 24 h, and proteins were extracted for co-immunoprecipitation with anti-SIRT1 and probed with anti-eNOS or anti-SIRT1. The bands for SIRT1-immunoprecipitated eNOS were scanned and normalized with GAPDH. All experiments were repeated independently three times. eNOS, endothelial nitric oxide synthase; SIRT1, sirtuin 1.
