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Sequences of the primers used for eNOS and SIRT1 detection by quantitative polymerase chain reaction.
| Gene | Primer sequence (5′-3′) |
|---|---|
| eNOS | Forward: TTCCTGGACATCACTTCCCC |
| Reverse: CTTCCATTCTTCGTAGCGCC | |
| SIRT1 | Forward: TGCCATCATGAAGCCAGAGA |
| Reverse: AACATCGCAGTCTCCAAGGA | |
| GAPDH | Forward: CGGAGTCAACGGATTTGGTCGTAT |
| Reverse: AGCCTTCTCCATGGTGGTGAAGAC |
eNOS, endothelial nitric oxide synthase; SIRT1, sirtuin 1.
