Figure 3.

(A and B) Targetscan software indicated that TUSC2 was a target gene of miR-663b. (B) The luciferase reporter vectors containing wild type (WT) or mutant type (MT) of TUSC2 3′UTR were generated. (C) The luciferase activity was significantly reduced in CNE1 cells co-transfected with the WT-TUSC2 luciferase reporter vector and miR-663b mimics, which was eliminated by transfection with MT-TUSC2 luciferase reporter vector. **P<0.01 vs. Control. TUSC2, tumor suppressor candidate 2; NC, negative control; WT, wildtype; MT, mutant; UTR, untranslated region.