Table 4.
Antimicrobial Data Against Wild Type and Engineered Strains of E coli Carrying Specific Resistance Determinants (MIC, μg/mL, (μM))a,b.
| Strain | BM13 (AG006) | AG007 | AG105 | AG009 | AG036 | AG037 |
|---|---|---|---|---|---|---|
| Resistance Mechanism | Wild type | AAC(3) | AAC(2′) | AAC(6′) | ANT(4′, 4″) | APH(3′, 5″) |
| Gentamicinc | 0.5 (1) | 32 (67) | 32 (67) | -d | 0.5 (1) | 0.25 (0.5) |
| Kanamycin Ac | 1–2 (2–4) | -d | 1–2 (2–4) | 128 (265) | 16 (33) | >256 (>530) |
| Tobramycinc | 0.5 (1) | -d | -d | 64 (137) | 16 (34) | 1 (2) |
| Neomycin 6 | 1 (2) | 4 (7) | 2 (3) | 8 (13) | 32 (52) | >256 (>417) |
| 22 | 1 (2) | 2 (3) | 2 (3) | 16 (24) | 32 (49) | >256 (>389) |
| 21 | 1 (2) | 2 (3) | 2 (3) | 2 (3) | 64 (97) | >256 (>389) |
| 28 | 4–8 (6–12) | 16 (12) | 4 (6) | 16 (12) | >64 (>24) | >256 (>389) |
| 30 | 1 (2) | 2 (3) | 2–4 (3–7) | 64 (104) | 128 (207) | >256 (>414) |
| 37 | 2–4 (3–6) | 8–16 (12–23) | 8 (12) | 16 (23) | 4 (6) | >256 (>374) |
| 11 | 0.5 (0.8) | 2 (3) | 2 (3) | 32–64 (50–100) | 0.5 (0.8) | >256 (>399) |
| 26 | 2 (3) | 8 (11) | -d | 8–16 (11–23) | >64 (>93) | >256 (>364) |
| 40 | 4 (5) | 8 (11) | -d | 16–32 (22–44) | 8 (11) | >256 (>351) |
a
All values were determined in duplicate using two dilution series.
b
MICs are given in μg/mL and in parentheses in μM.
c
These AGAs were used to verify the resistance phenotype type of the recombinant strains.
d
Not determined