Skip to main content
. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Traffic. 2017 Feb 28;18(4):218–231. doi: 10.1111/tra.12469

Figure 2.

Figure 2

DENV/DC-SIGN complexes translocate to CCS for endocytosis. A. Confocal imaging shows colocalization (white arrows) of DENV-AlexaFluor568 (red) and DC-SIGN (labeled with DCN46-AlexaFluor647; blue) in the same EGFP-clathrin-coated structures (green) on MX-DC-SIGN cell surfaces. Of all DENV colocalized with CCS, 73 ± 28 % (N=17 cells, ± SD) are coincident with DC-SIGN. Scale bar, 2 µm. B. Super-resolution GSD imaging shows that DENV-AlexaFluor488 (green), CCS (labeled with Clc mAb followed by goat anti-mouse IgG-AlexaFluor532; red), and DC-SIGN (labeled with DCN46-AlexaFluor647; blue) are colocalized on MX-DC-SIGN cells. Scale bar, 150 nm. C. Confocal imaging shows colocalization (white arrows) of DENV (labeled with 1M4 followed by goat anti-human IgG-AlexaFluor 488; green) and DC-SIGN (labeled with H-200 followed by goat anti-rabbit AlexaFluor647 Fab2; blue) in the same CCS (labeled with Clc mAb followed by goat anti-mouse IgG-AlexaFluor568; red) on primary DC surfaces. Of all DENV colocalized with CCS, 66 ± 20 % (N=22 cells, ± SD) are coincident with DC-SIGN. Scale bar, 5 µm. Inset panels (right) are shown in the same colors as the whole cell image panels (top row) with the merge of DENV with CCS in the left panel of the bottom row and the merge of all three colors shown in the middle panel of the bottom row; inset scale bar, 1 µm.