Fig. 4.

Higher expression of HtrA does not change host cell binding capabilities and IL-8 production by H. pylori. IPTG-induced or control H. pylori wild-type strain 26695 (Hp wt) and 26695 transformed with htrA ²⁶⁶⁹⁵ were grown for 24 h in BHI broth medium with 10% FCS. Polarized MKN-28 cells were then co-incubated with H. pylori for 8 h using an MOI of 50. The numbers of adherent bacteria were determined by adhesion assays and counting the CFU on GC agar plates. The concentrations of secreted chemokine IL-8 were quantified by standard ELISA. All infection assays were done in triplicates