Table 4.
Comparison of diagnostic results obtained by cytogenetic analysis and multi-fusion gene F-qRT-PCR screening
| Subtype | Cytogenetics | F-qRT-PCR | ||||||
|---|---|---|---|---|---|---|---|---|
| Cyto- | Cyto+ | % (+) | Translocation | PCR- | PCR+ | % (+) | Fusion gene | |
| M1 | 4 | 0% | none | 4 | 0% | None | ||
| AML M2 | 1 | none | 1 | None | ||||
| a 1 | 90.5% | none | 1 | 95.2% | RUNX1-RUNX1T1 | |||
| 17 | t(8;21)(q22;q22) | 17 | RUNX1-RUNX1T1 | |||||
| 1 | t(16;21)(q24;q22) | 1 | AML1-MDS/EVII/MTG16 | |||||
| 1 | del(4)(q12) | 1 | FIP1L1-PDGFRA | |||||
| AML M3 | 3 | none | 3 | None | ||||
| 43 | 93.5% | t(15;17)(q22;q12) | 43 | 93.5% | PML-RARα | |||
| AML M4 | 2 | none | 2 | None | ||||
| 1 | 33.3% | t(8;21)(q22;q22) | 1 | 33.3% | RUNX1-RUNX1T1 | |||
| AML M5 | 22 | none | 22 | None | ||||
| 2 | 12.0% | inv(16)(p13;q22) | 2 | 12.0% | CBFβ-MYH11 | |||
| 1 | t(9;11)(p22;q23) | 1 | MLL-AF9 | |||||
| AML M6 | 1 | none | 1 | None | ||||
| 1 | 50.0% | del(1p) | 1 | 50.0% | SIL-TAL1 | |||
| Undecided | 3 | 100.0% | inv(16)(p13;q22) | 3 | 100.0% | CBFβ-MYH11 | ||
| 3 | t(6;11)(q26;q23) | 3 | MLL-AF6/AF10/ELL/ENL | |||||
| 1 | del(1p) | 1 | SIL-TAL1 | |||||
| subtotal | 34 | 74 | 68.5% | 33 | 75 | 69.4% | ||
aBold print indicates cases with inconsistent results obtained using the two diagnostic methods