Table 3.
Study 3 - protocol of oral tolerance and systemic immunization –single dose BPA, juvenile challenge (11)
| Age of Wistar rats | |||||
|---|---|---|---|---|---|
| G15 to 21 d | 25 d | 32 d | 46 d | 53 d | |
| Day of oral tolerance induction | P0 | P7 | P21 | P28 | |
| OVA-tolerized | 0 or 5 μg BPA/kg/day | 20 mg OVA gavage | OVA in CFA 100 μg s.c. | OVA boost 100 μg s.c. | Euthanasia |
| OVA-immunized | 0 or 5 μg BPA/kg/day | Gavage, vehicle | OVA in CFA 100 μg s.c. | OVA boost 100 μg s.c. | Euthanasia |
Ménard et al., PloS one 2014 (11). G15: gestational day 15; P: protocol day; CFA: complete Freund Adjuvant; s.c.: subcutaneous