Skip to main content
. Author manuscript; available in PMC: 2017 Jul 25.
Published in final edited form as: Cold Spring Harb Protoc. 2016 Mar 1;2016(3):pdb.top079764. doi: 10.1101/pdb.top079764

Figure 1.

Figure 1

A) Wild type cells grown in EMMG with or without 150 mg/l leucine added. Cell lengths at division (± SEM, 200 cells) are shown. B) Calcofluor staining of wild type cells grown in medium indicated to a cell density of 1.5 × 106 cell/ml. C) Pheromone secretion by wild type h− cells. A diploid tester strain was mixed 100:1 with haploid h− cells on MSA. Iodine staining was used to visualize the halo of sporulating diploid cells around the pheromone-producing haploid cells.