Table 1.
Approximate generation times of healthy haploids according to media and nitrogen source, and cell dimensions according to ploidy.
| Approximate generation times of healthy haploids | ||
|---|---|---|
| Media | Temperature | Generation time |
| YES | 20°C | ~8 hr |
| 25°C | 3 hr | |
| 28°C | 2 hr 45 min | |
| 30°C | 2 hr 30 min | |
| 32°C | 2 hr 10 min | |
| 35°C | 2 hr | |
| EMM2 | 20°C | ~10 hr |
| 25°C | 4 hr 30 min | |
| 28°C | 3 hr 45 min | |
| 30°C | 3 hr | |
| 32°C | 2 hr 30 min | |
| 35°C | 2 hr 20 min | |
| Nitrogen-dependent generation times of healthy haploids | ||
| Nitrogen source | Temperature | Generation time |
| EMM2 (ammonium) | 28°C | 3 hr 45 min |
| EMMG | 28°C | 3 hr 30 min |
| (glutamic acid) | ||
| EMMP (proline) | 28°C | 4 hr 30 min |
| MSL (arginine) | 28°C | 3 hr 30 min |
| YES (complex) | 28°C | 2 hr 45 min |
| Cell dimensions | ||
| Ploidy | Cell length | Cell width (constant) |
| Haploid | 12–14 μm | ~3.5 μm |
| Diploid | 20–24 μm | ~4.5–5 μm |