JetValve biaxial stiffness as a function of biohybrid composition, collection angle, and location. (a) Increasing protein percentage within the biohybrid ratio of spun scaffolds decreased the low strain (0–10%) and high strain (10–20%) biaxial global stiffness of scaffolds (N=6 production runs per condition, N=5 native leaflets; *p<0.5, data presented as mean ± s.e.m. (b) Conduit samples comprised of 60/40 P4HB/Gelatin blends were stiffer than corresponding collection angle leaflet samples for both low and high strains (N=6 production runs per conditions; *p<0.5 between leaflet and conduit stiffness for the same collection angle, data presented as mean ± s.e.m.).